Effect of adipose-derived mesenchymal stem cell transplantation on inflammatory response and ventricular remodeling after myocardial infarction

doi:10.3969/j.issn.2095-4344.2014.06.013

Abstract

Abstract:

BACKGROUND: Whether adipose-derived mesenchymal stem cells are able to exert immunomodulatory effects in the treatment of myocardial infarction, as well as the best time, is less reported.

OBJECTIVE: To observe the effect of adipose-derived mesenchymal stem cells on inflammatory reaction and ventricular remodeling after myocardial infarction, and to explore the possible mechanisms of adipose-derived mesenchymal stem cells for the treatment of myocardial infarction.

METHODS: Enzyme digestion method was employed to isolate and culture rat adipose-derived mesenchymal stem cells. By ligation of the left anterior descending coronary artery, we established animal models of myocardial infarction in 40 rats. The rats were randomly divided into four groups: sham group, control group (injected high-glucose Dulbecco’s modified Eagle’s medium), 3-hour transplantation group (transplanted adipose-derived mesenchymal stem cells after 3 hours of myocardial infarction), 7-day transplantation group (transplanted adipose-derived mesenchymal stem cells after 7 days of myocardial infarction). After 14 days of operation, the levels of tumor necrosis factor-α and interleukin-10 in the plasma were detected by enzyme linked immunosorbent assay. After 28 days of operation, the left ventricular end diastolic diameter, left ventricular end systolic diameter, left ventricular ejection fraction and left ventricular fractional shortening were measured by echocardiography.

RESULTS AND CONCLUSION: Compared with the control group, in the 3-hour transplantation group and 7-day transplantation group, the levels of tumor necrosis factor-α were significantly lower (P < 0.01), and the levels of interleukin-10 were significantly higher (P < 0.01) at postoperative 14 days; the left ventricular end diastolic diameter and left ventricular end systolic diameter in the two transplantation groups were also significantly smaller (P < 0.05), but left ventricular ejection fraction and left ventricular fractional shortening were significantly elevated (P < 0.05), which was more apparent in the 3-hour transplantation group than the 7-day transplantation group. Adipose-derived mesenchymal stem cells transplantation in acute phase of myocardial infarction can suppress the inflammatory response, regulate the cytokine network equilibrium, and thus delay ventricular remodeling and improve cardiac function.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, abdominal fat, myocardial infarction, ventricular remodeling, cytokines, cell transplantation

CLC Number:

R394.2

Fan Yan, Wang Jian-jun, Wei Feng, Fan Xiao-hai, Ma Ai-qun. Effect of adipose-derived mesenchymal stem cell transplantation on inflammatory response and ventricular remodeling after myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 900-905.

Figures/Tables 2

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